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=== 3.0 Creating ROI summaries === Once you have the segmentation mapped to the subject's native functional space, you can create summaries of the functional activation. Eg,: {{{ mri_segstats --seg fbert.feat/reg/freesurfer/aparc+aseg.nii.gz --nonempty --ctab-default \ --in fbert.feat/stats/cope1.nii.gz --sum fbert.sum.txt }}} This will create fbert.sum.txt which will be a text file with a table of data. Each row will be a segmentation. The columns will contain various measures, including the number of functional voxels and the mean, stddev, min, max, and range of the cope over each ROI. |
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Mapping automatic segmentations to the functional space
To follow this exercise exactly be sure you've downloaded the [wiki:FsTutorial/Data tutorial data set] before you begin. If you choose not to download the data set you can follow these instructions on your own data, but you will have to substitute your own specific paths and subject names.
1.0 Mapping the segmentations
The cortical and subcortical segmentations automatically generated by freesurfer can be mapped into the functional space, which can be useful for doing region of interest (ROI) analysis. This can be done with aseg2feat:
aseg2feat --feat fbert.feat --aseg aparc+aseg
and This command will create fbert.feat/reg/freesurfer/aparc+aseg.nii.gz. These are segmentations, meaning that each voxel has an integer value that corresponds to a particular structure. The mapping from structure number to name is given in ${FREESURFER_HOME}/FreeSurferColorLUT.txt.
2.0 Creating binary masks
The segmentation for a particular structure can be extracted to create a binary mask (i.e., a volume where the voxel value is 1 if it is in the structure and 0 otherwise). To make a binary mask of the left putamen, which has been assigned label 12 (see ${FREESURFER_HOME}/FreeSurferColorLUT.txt), use the following command:
avwmaths ./fbert.feat/reg/freesurfer/aparc+aseg.nii.gz \ -thr 12 -uthr 12 \ ./fbert.feat/reg/freesurfer/lh.putamen.nii.gz
To view this binary mask on the anatomical:
tkmedit bert orig.mgz -aux brain.mgz \ -overlay ./fbert.feat/reg/freesurfer/lh.putamen.nii.gz \ -overlay-reg ./fbert.feat/reg/freesurfer/anat2exf.register.dat \ -fthresh 0.5 -fmid 1 -fslope 1 \ -segmentation ${SUBJECTS_DIR}/bert/mri/aparc+aseg.mgz \ ${FREESURFER_HOME}/FreeSurferColorLUT.txt
You should see the image below:BR attachment:tkm-lh.putamen-cor-128-small.jpg
3.0 Creating ROI summaries
Once you have the segmentation mapped to the subject's native functional space, you can create summaries of the functional activation. Eg,:
mri_segstats --seg fbert.feat/reg/freesurfer/aparc+aseg.nii.gz --nonempty --ctab-default \ --in fbert.feat/stats/cope1.nii.gz --sum fbert.sum.txt
This will create fbert.sum.txt which will be a text file with a table of data. Each row will be a segmentation. The columns will contain various measures, including the number of functional voxels and the mean, stddev, min, max, and range of the cope over each ROI.